Antibodies to renin as proof of the pathogenesis of sustained renal hypertension.

Antirenin to hog renin produced in the dog neutralizes the acute pressor effect of dog as well as hog renin. A course of intramuscular injections of hog renin in chronic renal hypertensive dogs produces an antihypertensive effect which is correlated with the antirenin titers of the plasma. Passive transfer of high titer homologous antiserum to hog renin produces an anti-hypertensive effect in chronic renal hypertensive dogs which is correlated with the antirenin titer. The results suggest a pathogenetic role for renin in experimental renal hypertension in the dog. IN VIEWT of the fact that reniu is a protein, we thought it possible to study the patho-genesis of experimental renal hypertension by producing an antibody to renin. In figure 1 is seen the typical pressor effect of what would be 2 units of hog renin injected intravenously after mixing with 1 ml. of normal dog serum.1' 2 The middle of the figure is the result of the intravenous injection into this bilaterally nephrectomized, etherized dog of 2 units of hog renin mixed with 1 ml. of serum from a dog previously repeatedly injected with crude hog renin. The right of the figure shows the result of another control injection of 2 units of hog renin mixed with 1 ml. of normal dog serum. It should be noted that there are approximately 2 antirenin units per ml. of plasma or serum. We found that antirenin to hog renin not only neutralizes hog renin but also dog renin, as mentioned by Dr. Hel-mer. We also found that it does not neutralize human, monkey, or other primate renins. a FIG. 1. Abscissa, time in minutes; ordinate, blood pressure (mnm. Hg). 1. Intravenous injection of approximately 2 dog units of crude hog renin mixed with 1 ml. of normal dog serum. 2. Intravenous injection of approximately 2 dog units of crude hog renin mixed with antiserum from a dog repeatedly injected with crude hog reain. 3. Same as 1. (Re